Chapter 4, Page 2

How is the test performed in the laboratory?

After waiting for about 30 minutes after ejaculation, to allow the semen to liquefy, the doctor will check the semen for:

The volume of the ejaculate.

While a lot of men feel their semen is "too little or not enough", abnormalities of volume are not very common. They usually reflect a problem with the accessory glands - the seminal vesicles and prostate - which are what produce the seminal fluid. Normal volume is about 2 to 6 ml. A low volume (less than 1 ml) is an uncommon problem, and is often due to incomplete ejaculation (which is not rare because of the stress of producing a sample in the lab!) or spillage. Since the major portion of the ejaculate is produced in the seminal vesicles, a persistently low volume is because of a problem with seminal vesicle function - either absence of the seminal vesicles, or an ejaculatory duct obstruction. A very high volume surprisingly will also cause problems, because this dilutes the total sperm present, decreasing their concentration.

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The viscosity.

During ejaculation the semen spurts out as a liquid that gels promptly. This should liquefy again in about 30 minutes to allow the sperm free motility. If it fails to do so, or if it is very thick in consistency even after liquefaction, this suggests a problem most usually one of infection of the seminal vesicles and prostate.

The pH.

Normally the pH of semen is alkaline because of the seminal vesicle secretion. An alkaline pH protects the sperm from the acidity of the vaginal fluid. An acidic pH suggests problems with seminal vesicle function, and is usually found in association with a low volume of the ejaculate and the absence of fructose.

The presence of a sugar called fructose.

This sugar is produced by the seminal vesicles and provides energy for sperm motility. Its absence suggests a block in the male reproductive tract at the level of the ejaculatory duct. The most important test is the visual examination of the sample under the microscope.

What do sperm look like? Sperm are microscopic creatures that look like tiny tadpoles swimming about at a frantic pace. Each sperm has a head, which contains the genetic material of the father in its nucleus; and a tail that lashes back and forth to propel the sperm along. The mid-piece of the sperm contains mitochondria, (the power house of the sperm) which provide the energy for sperm motion.

Ask to see the sperm sample for yourself under the microscope - if normal, the sight of all those sperm swimming around can be very reassuring. You are likely to be awestruck by the massive numbers and the frenzy of activity. If the test is abnormal, seeing for yourself gives you a much better idea of what the problem is! A good lab should be willing to show you, and to explain the problem to you.


Fig 1. Sperm as seen under a microscope.


Fig 2. The anatomy of a sperm.

Credits: How to Have a Baby: Overcoming Infertility