Chapter 24, Page 4

Sperm processing

Sperm processing allows the doctor to concentrate the actively motile sperms into a small volume of culture fluid. Sperm do not remain alive in the culture medium for very long unless maintained at the right conditions - hence a prompt insemination after sperm processing is important. This is why processing should preferably be done in the clinic itself, so that time is not wasted in transporting the sperm after the wash.

Laboratory Techniques

There are different methods of processing the sperm, and all of these require special laboratory expertise.

  • The simplest method is that of washing the semen with a culture medium (by centrifuging it and collecting the pellet) but this is a poor technique and is not recommended.
  • The swim-up method uses a layering technique, in which a special culture medium is placed above the semen in a test-tube. The good quality sperm will swim up into the culture medium; and after 45 to 60 minutes, this medium (with the motile sperm) is removed and injected into the uterine cavity.
  • The more sophisticated methods today use a density gradient column. This method allows one to separate the good quality sperm from the immotile sperm, the pus cells and the seminal plasma, because these are lighter than the motile sperm. It provides the best recovery of motile sperm and is the standard technique in use today, especially for poor quality sperm samples.

Recent advances

Of late, doctors have tried adding various chemicals to the washed sperm to try to improve their motility, so as to increase the chances of their reaching their goal. These chemicals include caffeine and pentoxyfylline and they may be helpful in some patients.

During IUI, sperm are injected into the uterine cavity in the hope that they will then swim up from here into the fallopian tubes where they can fertilize the egg. But then, why not inject the sperm direct into the fallopian tubes where the eggs is present? This feat was technically difficult to accomplish in the past, because the tubes are so thin. Today, with specially designed catheters (Jansen-Anderson catheter sets), it is possible to do this in the doctor's clinic. Thus, the processed sperm can be injected directly into the tubes under ultrasound guidance, without anesthesia or surgery! This is an intratubal insemination - also known as a SIFT - (sperm intrafallopian transfer).

Credits: How to Have a Baby: Overcoming Infertility

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