Chapter 25, Page 5

Insemination, Fertilization, and Embryo Culture

The aspirated follicular fluid is then immediately carried into the laboratory (which is adjoining the operation theatre) where it is examined by the embryologist under a stereozoom microscope, in order to identify the egg. Each egg is surrounded by sticky cumulus cells, and is called an oocyte-cumulus complex.

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These are washed in medium, graded for their maturity and then transferred into the CO2 incubator. The maturity of an egg determines when the sperm will be added to it (insemination). Insemination can be done immediately upon harvest, but is usually done after 2-6 hours.


Fig 2. Checking the eggs under the stereozoom microscope in the IVF lab

Credits: How to Have a Baby: Overcoming Infertility